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mtp format  (Greiner Bio)

 
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    Greiner Bio mtp format
    Mtp Format, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 96/100, based on 264 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mtp format/product/Greiner Bio
    Average 96 stars, based on 264 article reviews
    mtp format - by Bioz Stars, 2026-03
    96/100 stars

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    T m data from a 96‐well MTP made up of 92 clones of the saturation library DERA Lb Q54X, two wild‐type controls (wt) and two negative controls (neg) lacking the gene coding for DERA Lb and thus not giving a corresponding melting curve. The red bars correspond to the T m values ±1 standard deviation from triplicate determinations. From the entire set 32 clones were sequenced, and these bars are labelled with the amino acid found at position 54. The high reproducibility of triplicate measurements, the good agreement of identical variants and the high yield of positively determined T m data demonstrate the high sample quality and the reliability of the method.

    Journal: The Febs Journal

    Article Title: nanoDSF as screening tool for enzyme libraries and biotechnology development

    doi: 10.1111/febs.14696

    Figure Lengend Snippet: T m data from a 96‐well MTP made up of 92 clones of the saturation library DERA Lb Q54X, two wild‐type controls (wt) and two negative controls (neg) lacking the gene coding for DERA Lb and thus not giving a corresponding melting curve. The red bars correspond to the T m values ±1 standard deviation from triplicate determinations. From the entire set 32 clones were sequenced, and these bars are labelled with the amino acid found at position 54. The high reproducibility of triplicate measurements, the good agreement of identical variants and the high yield of positively determined T m data demonstrate the high sample quality and the reliability of the method.

    Article Snippet: The phylogenetically diverse 2‐deoxyribose‐5‐phosphate (DERA) library was provided as lyophilised cell‐free extract in a commercial MTP format (Prozomix Limited, Haltwhistle, UK).

    Techniques: Clone Assay, Standard Deviation